Estimating of Cells Processes in Endometrial Hyperplasia in Women with Extragenital Pathology
Changes at the cellular and subcellular levels evolving in hyperplastic endometrium in women with extragenital pathology were estimated. This enabled further evaluation of the treatment efficacy. The study involved 105 women with endometrial hyperplasia and extragenital pathology in perimenopausal period. Diagnostics included general clinical examinations, the study of sex and gonadotropic hormones levels in blood serum, lipid and carbohydrate metabolism indices, transabdominal and transvaginal sonography, endoscopic examination using “Karl Storz” heteroscope and endometrial biopsy with followed histological examination of tissues from uterus cavity. The activity of intracellular ATP transport system of endoplasmic reticulum of endometrial cells was assessed according to the difference of inorganic phosphorus content determined by Fiske-Subbarow method.
Results revealed the presence of correlation between histopathological characteristics of endometrium and changes of Ca2+, Mg2+ - ATP-activity of endoplasmic reticulum in the application of progestogens in women with endometrial hyperplasia. The highest level of Ca2+, Mg2+ - ATP-activity was determined in cases of atypical endometrial hyperplasia. The activity of these indicators decreased in the following sequence: focal adenomatosis, nonatypical complex hyperplasia, simple nonatypical hyperplasia, endometrial polyps, phase of proliferation and secretion. Presence of glandular component in polyps was characterized by increase in Ca2 +, Mg2 + - ATP activity, the presence of fibrous component was accompanied by their decrease. Therefore, reduction of Ca2+, Mg2+ - ATP-activity and positive morphological transformation of the endometrium during progestin therapy in women with endometrial hyperplasia indicate the efficacy and can be considered as prognostic criteria.
Babych LG. Membranes mechanisms of regulation of Са ions concentration in smooth muscle cells. Ukrainskyi biokhimichnyi zhurnal. 1999; 75(5): 10-19.
Sukhikh GT, Serov VN, Dementieva MM, Kolobova EA. Activity of Са2+/ Mg2+_ dependendant endonuclease as a biological mark of apoptosis in the hyperplastic processes and cancer of endometrium. Akusherstvo i ginekologiya. 2000; 4: 41-45.
Tatarchuk TF, Pedachenko NU, Khominska ZB. Metabolic syndrome and hyperproliferative processes of endometrium. Reproduktivnaya endokrinologiya. 2014; 2(16): 61-72.
Shymanska B, Gardyshevska A, Pabikh Y, Chaikovsky K. Endometrial hyperplasia: efficacy of treatment due to natural micronizative progesteron with application into vagina. Obzor menopauzalnyi. 2006; 2: 75-79.
Clark TJ., Voit D, Gupta JK et al. Accuracy of hysteroscopy in the diagnosis of endometrial cancer and hyperplasia: a systematic quantitative review. J Am Med Assoc. 2002; 288: 1610-1621.
Goldstein SR, Monteagudo A, Popiolek D et al. Evaluation of endometrial polyps. Am J Obstet Gynecol. 2002; 186: 669-674
Okaro E, Condous G, Bourne T. The role of transvaginal ultrasound in the management of abnormal uterine bleeding. Gynecological Surgery. 2004; 1(2): 119-126.
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