Changes in the Metabolism of Nitric Oxide, Hydrogen Sulfide of the Oral Fluid Taking into Account Age and Dental Status

DOI: https://doi.org/10.21802/acm.2022.1.8
Keywords: Nitric oxide, Nitrites, Nitrates, L-arginine, Arginase, Hydrogen sulfide, Oral fluid, Dental status

Abstract

The relevance of the study is conditioned by the prevalence of dental pathology, its complicated course in senior age and the search for the age-associated metabolic markers of oral fluid.

Objective. The aim of the study is: to determine the age peculiarities of the content of nitric oxide and hydrogen sulfide metabolism in the oral fluid of patients, taking into account their dental status.

Materials and Methods. Indicators of nitric oxide metabolism, L-arginine/arginase, hydrogen sulfide content in oral fluid, dental status of different aged people under conditions of intact dentitions, their defects and against the background of correction with different types of dentures, were studied.

Results. It was determined that with age, changes in oral fluid are characterized by an increase in nitrite ion, the amount of nitrites and nitrates, increasing the formation of peroxynitrite against the background of lower levels of L-arginine and hydrogen sulfide, inhibition of arginase activity. The results obtained are confirmed by the presence of close correlations between the concentrations of hydrogen sulfide and peroxynitrite (reverse, strong), caries intensity and: the total level of nitrites and nitrates (direct, strong), peroxynitrite content (direct, medium strength).

Conclusion. The studied biomarkers of oral fluid depend more on age, hygienic condition of the oral cavity, intensity of dental caries and defects of dentitions than types of dentures. Therefore, age-related changes in the biochemical composition of oral fluid, metabolic, somatic, comorbid pathologies are likely to be a trigger for structural-functional disorders of the dental-maxillofacial system.

References

Leibyuk LV, Rozhko MM, Dmutrushun TM. Kharakterystyka reolohichnykh vlastyvostey rotovoyi ridyny u khvorykh na tsukrovyy diabet, yaki korystuyutʹsya povnymy znimnymy plastynkovymy protezamy. Klinichna Stomatolohiya. 2018;3:24-33. [in Ukrainian]. Available from: https://doi.org/10.11603/2311-9624.2018.3.9338

Polishchuk TV, Trufanova VP, Padalka AI, Sheshukova OV. Kharakterystyka urazhenosti kariyesom ditey ta pidlitkiv. Visnyk stomatolohiyi. 2014;3:108-11. [in Ukrainian].

Kovalishin AYu., Rozhko MM, Dmitrishin TM. Osoblyvosti funktsionalʹnoho stanu zuboshchelepnoyi systemy u patsiyentiv, shcho korystuyutʹsya neznimnymy ta znimnymy ortopedychnymy konstruktsiyamy. Visnyk problem biolohiyi i medytsyny. 2019;4(2):377-382. [in Ukrainian].

Kalashnikov DV, Kindiy DD, Korol DM, Kindiy VD. Analysis of denture base impact on the denture foundation area tissues. Svit medytsyny ta biolohiyi. 2020; 1(71):58-62. Available from: https://doi.org/10.26724/2079-8334-2020-1-71-58-62

Mykhaylenko TM, Erstenyuk HM, Rozhko MM. Biokhimichni pokaznyky rotovoyi ridyny u osib, shcho korystuyutʹsya znimnymy konstruktsiyamy zubnykh proteziv ta mayutʹ riznyy rivenʹ hihiyeny rotovoyi porozhnyny. Visnyk stomatolohiyi. 2014;2:49-53. [in Ukrainian].

Pavlenko ÉM. Metabolicheskiye izmeneniya v rotovoy zhidkosti u lits pozhilogo i starcheskogo vozrasta pri generalizovannom parodontite. Visnyk problem biolohiyi i medytsyny. 2017;2:382-6.

Potapchuk AM, Onipko Ye.L., Sabov MYu., Almashi VM, Yurzhenko AV., Stecique MO. Biomarkery v diahnostytsi khvorob parodonta. Klinichna stomatolohiya. 2019;2:19-25. [in Ukrainian]. Available from: https://doi.org/10.11603/2311-9624.2019.2.10395

Zubachyk VM, Yarychkivska NV. Rolʹ oksydu azotu v homeostazi tkanyn parodonta (ohlyad literatury). Bukovynsʹkyy medychnyy visnyk. 2016;2(78):194-8. [in Ukrainian].

Parwani SR, Chitnis PJ, Parwani RN. Salivary nitric oxide levels in inflammatory periodontal disease - a casecontrol and interventional study. International Journal of Dental Hygiene. 2012;10:67-73. Available from: https://doi.org/10.1111/j.1601-5037.2011.00508.x

Lahoda LS. Hihiyenichnyy stan porozhnyny rota u ditey, yaki prozhyvayutʹ v riznykh ekolohichnykh umovakh. Klinichna stomatolohiya. 2018;2:76-82. [in Ukrainian]. Available from: https://doi.org/10.11603/2311-9624.2018.2.8813

Vivcharenko TI, Rozhko MM. Otsinka stanu tkanyn parodonta v patsiyentiv z heneralizovanym parodontytom ta hipertonichnoyu khvoroboyu. Halytsʹkyy likarsʹkyy visnyk. 2017;24(2):13-5. [in Ukrainian]. Available from: https://doi.org/10.21802/gmj.2017.2.10

Ivanochko RB, Biletsʹka LP, Sklyarov OYA. Zminy pokaznykiv systemy L-arhinin-nitrohenu oksydarhinaza ta oksydatyvnykh protsesiv u plazmi krovi khvorykh z khronichnoyu nyrkovoyu nedostatnistyu do ta pislya hemodializu. Eksperymentalʹna ta klinichna fiziolohiya i biokhimiya. 2014;1:66-70. [in Ukrainian].

Dombkowski RA, Naylor MG, Shoemaker E, Smith M, DeLeon ER, Stoy GF, Gao Y, Olson KR. Hydrogen sulfide (H₂S) and hypoxia inhibit salmonid gastrointestinal motility: evidence for H₂S as an oxygen sensor. Journal of Experimental Biology. 2011 Dec 1;214(Pt 23):4030-40. Available from: https://doi.org/10.1242/jeb.061473

Kaminskyy DV, Kryshchyshyn AP, Yelisyeyeva OP, Lesyk RB. Donory H2S u stvorenni innovatsiynykh likarsʹkykh zasobiv. Zhurnal orhanichnoyi ta farmatsevtychnoyi khimiyi. 2017;2(58):27-46. [In Ukrainian]. Available from: https://doi.org/10.24959/ophcj.17.918

Published
2022-10-17
Issue
Vol. 28 No. 1 (2022)
Section
Original Research

Copyright (c) 2022 Oleh Barabash, Nataliia Voronych-Semchenko, Taras Kryvenkyi

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