Keywords
taxonomic composition
population level
How to Cite
Abstract
The article considers the taxonomic composition and the population level of the oral cavity microbiota and changes in their characteristics after prosthetic rehabilitation with partial removable dentures. In the patients with partial loss of teeth wearing conventional removable dentures, the taxonomic composition of the gingiva microbiota changes due to the biotope contamination with opportunistic S. aureus and yeast-like fungi of the Candida genus, S. haemolyticus, K. pneumoniae, enterobacteria (E.coli, K.pneumoniae, E.cloacae), B.catarralis, M.lacunata and partial elimination of S. sialivarius, S.sanguis, S. mutans, S. mitis from the biota.
The objective of the research was to study changes in the gingiva in the examined patients.
Materials and Methods. Microbiological (bacteriological and mycotic) examination of gum mucus secretion in the patients with partial loss of teeth was performed. The control group consisted of 50 (23 men and 27 women) patients without any infectious and noncommunicable diseases, who had no problems adapting to removable denture during the last 6 months.
Results and Discussion. In 78.72% of the patients, microorganisms isolated and identified from the surface of the prosthetic bed, were in associations that consisted of 2 (31.70%), 3 (8.51%) and 4 (8.51 %) taxons. The most numerous associations consisting of 2 taxons were: S. aureus and M. lacunata; S. aureus and N. flavescens; S. haemolyticus and B.catarrhalis as well as C. albicans and S. epidermidis. In the biotope (the surface of the gum mucosa of the prosthetic bed) of the patients wearing partial removable laminar dentures, the population level reduced in S. salivarius, S. sanguis, S. mutans, S. mitis and significantly increased in opportunistic staphylococci (S. aureus, S. haemolyties, S. epidermidis), S. pyogenes, enterobacteria (E. coli, K. pneumoniae, E. clocacae), B.catarrhalis. Microorganisms S. aureus and C. albicans reached a high population level; it increased in S. haemoliticus, S. epidermidis, S. pyogenes, E. coli, E. cloacae, K. pneumoniae as well.
Conclusions. In the patients with partial loss of teeth wearing conventional removable dentures, the taxonomic composition of the gingiva microbiota changed due to the contamination of the habitat with opportunistic S. aureus and yeast-like fungi of the Candida genus, S. haemolyticus, K. pneumoniae, enterobacteria (E .coli, K. pneumoniae, E. cloacae), B. catarralis, M. lacunata and partial elimination of S. sialivarius, S.sanguis, S. mutans, S. mitis from the biota.
References
Pietiäinen M, Liljestrand JM, Kopra E et al. Mediators between oral dysbiosis and cardiovascular diseases. Eur J Oral Sci. 2018;126(1):26-36. DOI: https://doi.org/10.1111/eos.12423 [PMid:30178551]
Zhang M, Zheng Y, Li Y et al. Acid-resistant genes of oral plaque microbiome from the functional metagenomics. J Oral Microbiol. 2018;10(1):1424455. DOI: https://doi.org/10.1080/20002297.2018.1424455 [PMid:29503702 PMCid:PMC5795652]
Zheng X, He J, Wang L et al. Ecological effect of arginine on oral microbiota. Scientific Reports. 2017;7:7206. doi: 10.1038 / s41598-017-07042-w
Vesty A, Biswas K, Taylor MW et al. Evaluating the impact of DNA extraction method on the representation of human oral bacterial and fungal communities. PLoS One. 2017;12(1):e0169877. DOI: https://doi.org/10.1371/journal.pone.0169877 [PMid:28099455 PMCid:PMC5242530]
Kilian M, Chapple IL, Hannig M et al. The oral microbiome - an update for oral healthcare professionals. Br Dent J. 2016;221(10):657-666. DOI: https://doi.org/10.1038/sj.bdj.2016.865 [PMid:27857087]
Safarov AM. Mikrobnaya obsemenennost polosti rta pri nosheniyi syemnykh zubnykh protezov na osnove razlichnykh materialov. Sovremennaya stomatologiya. 2010;2:103-105.
Sokolova II, Skidan KV, Voropaeva LV et al. Mikroflora polosti rta, disbakterioz i puti yego korrektsiyi probiotikami. Eksperymenalna i klinichna medytsyna. 2010;2:64-69.
Tsarev VN. Mikrobiologiya, virusologiya, immunologiya polosti rta: uchebnik. Tsarev VN, editor. Moscow: GEOTAR-Media; c2013. 576 p.
Lewandowsky RA. Species composition and population level of the mouth microflora in people with complex maxillofacial pathology when using different types of prosthetic devices and its sensitivity to antiseptic preparations in Vitro. Clinical & Experimental Pathology. 2014;13(1):69-74. [published in Ukrainian]
Burdeniuk IP, Lewandowsky RA, Maksymiv OO et al. Aerobna ta fakultatyvno aerobna mikroflora rotovoii porozhnyny liudei zi skladnoiu shchelepno-lytsevoiu patolohiieiu, yaki korystuiutsia riznymy vydamy pokryvnykh, rezektsiinykh ta povnykh znimnykh proteziv ta vybirkova yii chutlyvist do dii deiakykh antyseptychnykh preparativ. Malanchuk VO, editor. Materialy VI Ukrainskoho Mizhnar. konhresu, prysviach. 100-richchiu z dnia narodzhennia prof. H.I. Semenchenko ta 95-littiu kafedry khirurhichnoii stomatolohii ta shchelepno-lytsevoii khirurhii. Stomatolohiia. Implantolohiia. Osteointehratsiia; 2014; Kyiv. 299-305.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.