DNA Fingerprinting of Resistant Mycobacterium tuberculosis Isolates in Iran by IS6110-RFLP Method
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Keywords

drug resistance
genetic fingerprinting
pulmonary tuberculosis

How to Cite

Farazi, A., Sofian, M., Keshavarz, R., & Malekmohammadi, S. (2019). DNA Fingerprinting of Resistant Mycobacterium tuberculosis Isolates in Iran by IS6110-RFLP Method. Galician Medical Journal, 26(3). https://doi.org/10.21802/gmj.2019.3.1

Abstract

The objective of the research was to identify resistant Mycobacterium tuberculosis strains and recognize their molecular epidemiology and how they are disseminated in order to determine the cause and the effect of drug resistance and the process of its development.

         Materials and methods. Genomic deoxyribonucleic acid obtained from 37 drug-resistant Mycobacterium tuberculosis isolates were examined and fingerprinted by the IS6110- restriction fragment length polymorphism method. The data obtained were then analyzed using SPSS statistics software.

         Results. The mean patients’ age was 51 ± 15.5 years. There were 46% of male patients, 67.6% of the patients from urban areas, 86.5% of Iranians, 21.6% of relapsed cases, 8.1% of human immunodeficiency virus-positive patients, and 10.8% of the patients with a history of contact with tuberculosis patient. Based on IS6110 - restriction fragment length polymorphism, 30 different genetic types were observed which indicated a significant variation of this pathogen in Markazi province, Iran. The number of cluster genotypes was determined by 6 clusters; the number of unique types was 24. There were no relationships between age, gender, nationality, residence, close contact with tuberculosis patients, recurrence of tuberculosis, positive human immunodeficiency virus status and clustered or non-clustered strain genotype.

         Conclusions. Considering the high genetic diversity in Mycobacterium tuberculosis strains, it can be concluded that, based on IS6110 - restriction fragment length polymorphism, about 65% of cases occurred due to reactivation, and 35% of the cases were due to recent transmission. The information obtained through the molecular typing method can be very effective in future planning for tuberculosis control in Iran.

https://doi.org/10.21802/gmj.2019.3.1
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References

Global tuberculosis report 2018. Geneva: World Health Organization; 2018. ISBN 978-92-4-156564-6. Available from: www.who.int/tb/publications/global_report/en/.

Guidelines for surveillance of drug resistance in tuberculosis - 5th ed. Available from: www.who.int/tb/publications/2015/drs_guidelines/en/.

Guidelines for treatment of drug-susceptible tuberculosis and patient care, 2017 update. Geneva: World Health Organization; 2017. Available from: https://apps.who.int/iris/bitstream/handle/10665/255052/9789241550000-eng.pdf

Farazi A, Sofian M, Zarrinfan N et al. Drug Resistance Patterns and Associated Risk Factors of Tuberculosis in the Central Province of Iran. Caspian J Intern Med. 2013; 4(4):785-789.

Nasiri MJ, Dabiri H, Darban-Sarokhalil D et al. Prevalence of drug-resistant tuberculosis in Iran: systematic review and meta-analysis. Am J Infect Control. 2014;42(11):1212-1218. DOI: https://doi.org/10.1016/j.ajic.2014.07.017 [PMid:25242634]

Gandhi NR, Nunn P, Dheda K et al. Multidrug-resistant and extensively drug-resistant tuberculosis: a threat to global control of tuberculosis. Lancet. 2010;375(9728):1830-1843. DOI: https://doi.org/10.1016/S0140-6736(10)60410-2

Joseph BV, Soman S, Radhakrishnan I et al. Molecular epidemiology of Mycobacterium tuberculosis isolates from Kerala, India using IS6110-RFLP, spoligotyping and MIRU-VNTRs. Infect Genet Evol. 2013;16:157-164. DOI: https://doi.org/10.1016/j.meegid.2013.01.012 [PMid:23395649]

Bouklata N, Supply P, Jaouhari S et al. Molecular typing of Mycobacterium tuberculosis complex by 24-locus based MIRU-VNTR typing in conjunction with spoligotyping to assess genetic diversity of strains circulating in Morocco. PLoS One. 2015;10(8):e0135695. DOI: https://doi.org/10.1371/journal.pone.0135695 [PMid:26285026 PMCid:PMC4540494]

Asgharzadeh M, Shahbabian K, Majidi J et al. IS6110 restriction fragment length polymorphism typing of Mycobacterium tuberculosis isolates from East Azerbaijan Province of Iran. Mem Inst Oswaldo Cruz. 2006;101(5):517-521. DOI: https://doi.org/10.1590/S0074-02762006000500006 [PMid:17072455]

Global Laboratory Initiative. Mycobacteriology Laboratory Manual. Stop TB Partnership. 2014. Available from: www.who.int/tb/laboratory/mycobacteriology-laboratory-manual.pdf

Performance Standards for Antimicrobial Disc Susceptibility Tests. Approved Standard. 11th Edition. Wayne, Pennsylvania, USA. 2012;32(1).

Farina P, Nourouzi J, Kargar M et al. Study on epidemiological patterns of mycobacterium tuberculosis by fingerprinting. Med J I.R. Iran. 2008; 6(21):59-64.

Asgharzadeh M, Alibakhshi A, Ranjbari J et al. Study role of age in contact dependent transmission of mycobacterium tuberculosis in northwest of Iran by IS6110-RFLP method. Modares Journal of Medical Sciences. 2009;12(1):11-16.

Gutiérrez MC, Vincent V, Aubert D et al. Molecular fingerprinting of Mycobacterium tuberculosis and risk factors for tuberculosis transmission in Paris, France, and surrounding area. J Clin Microbiol. 1998; 36(2):486-492.

Farnia P, Mohammadi F, Masjedi MR et al. Evaluation of tuberculosis transmission in Tehran using RFLP and spoligotyping methods. J Infect. 2004;49(2):94-101. DOI: https://doi.org/10.1016/j.jinf.2003.11.015 [PMid:15236915]

Richardson M, van der Spuy GD, Sampson SL et al. Stability of polymorphic GC-rich repeat sequence-containing regions of Mycobacterium tuberculosis. J Clin Microbiol. 2004;42(3):1302-1304. DOI: https://doi.org/10.1128/JCM.42.3.1302-1304.2004 [PMid:15004103 PMCid:PMC356821]

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