Abstract
The objective of this research was to determine the import of Justicia flava methanolic extract against petroleum-stimulated reduced cell division and DNA fragmentation.
Materials and Methods. Ninety rats were distributed and treated as follows for thirty days: Group A - normal diet; Group B - normal feed + 100mg/kg bwt of Justicia flava methanolic extract; Group C - normal feed + 200mg/kg bwt of Justicia flava methanolic extract; Group D - normal feed + 300mg/kg bwt of Justicia flava methanolic extract; Group E - normal feed + 250 mg/kg bwt of standard ascorbic acid; Group F - crude oil-tainted feed; Group G - crude oil-tainted feed + 250 mg/kg bwt of standard ascorbic acid; Group H - crude oil-tainted feed + 100mg/kg bwt of Justicia flava methanolic extract; Group I - crude oil-tainted feed + 200mg/kg bwt of Justicia flava methanolic extract. After thirty days, the rats were sacrificed under chloroform sedation and the liver and kidney were collected for cellular analysis via standard protocols.
Results. The results showed that administration of Justicia flava methanolic extract decreased petroleum-induced reduced cell division and DNA damage in the liver and kidney of experimental rats. Justicia flava methanolic extract is potent antidote for petroleum-induced DNA damage in the liver and kidney of rats.
Conclusions. It is no doubt that ingestion of petroleum-tainted diet could culminate in DNA damage in organs and tissues of animals. However, the administration of Justicia flava methanolic extract was found to prevent this damage in renal and liver cells of experimental rats.
References
Kponee KZ, Chiger A, Kakulu II et al. Petroleum contaminated water and health symptoms: a cross-sectional pilot study in a rural Nigerian community. Environmental Health. 2015;14:86. DOI: https://doi.org/10.1186/s12940-015-0073-0 [PMid:26546277 PMCid:PMC4636824]
Nriagu J, Udofia EA, Ekong I et al. Health risks associated with oil pollution in the Niger Delta, Nigeria. International Journal of Environmental Research and Public Health. 2016;13:346. DOI: https://doi.org/10.3390/ijerph13030346 [PMid:27007391 PMCid:PMC4809009]
Thompson LA, Darwish WS. Environmental chemical contaminants in food: Review of a global problem. J Toxicol. 2019;2019:2345283. DOI: https://doi.org/10.1155/2019/2345283 [PMid:30693025 PMCid:PMC6332928]
Gay J, Shepherd O, Thyden M et al. The Health Effects of Oil Contamination: A Compilation of Research. Worcester Polytechnic Institute Publication. c2019; 198p.
Achuba FI. Powdered Oil Palm (Elaesis guineensis Jacq) leaf as remedy for hydrocarbon induced liver damage in Rats. Nigerian Journal of Pharmaceutical and Applied Science Research. 2018a;7(3):89-95.
Achuba FI. Protective Influence of Elaesis guineensis leaf in diet on petroleum-mediated kidney damage in rat. Nigerian Journal of Pharmaceutical and Applied Science Research. 2018;7(2):33-38.
Sule OJ, Godwin J, Nnopu IA. Biochemical Investigation of Hepatotoxic effects of Antiretroviral Drugs on Wistar Albino Rats. Journal of Physiology and Pharmacology Advances. 2012;2(4):171-175.
Achuba FI, Ogwumu MD. Possible protective role of palm oil and beef liver on the kidney and liver of Wistar albino rats fed diesel-contaminated diet. Biokemistri. 2014;26(4):124-129.
Okpoghono J, Achuba FI, George BO. Protective effects of Monodora myristica extracts on crude petroleum oil-contaminated catfish (Clarias gariepinus) diet in rats. International Journal of Veterinary Science and Medicine. 2018;6:117-122. DOI: https://doi.org/10.1016/j.ijvsm.2018.03.006 [PMid:30255088 PMCid:PMC6146418]
Achuba FI. Evaluation of the protective effects of bitter leaf (Vernonia amygdalina) on haematological indices of rats fed with crude oil treated diet. Journal of Applied Sciences and Environmental Management. 2018;22(4):489-492. DOI: https://doi.org/10.4314/jasem.v22i4.8
Okoye JO, Ude T, Ibekailo SN et al. Histopathological, hemochromatotic, hypercholesterolemic, and androgenic effects of escravos crude oil on the testis in male chinchilla rabbits. British Biotechnology Journal. 2014;4(6):649-658. DOI: https://doi.org/10.9734/BBJ/2014/10808
Achuba FI. Effect of Moringa oleifera on crude oil mediated haematotoxicity in Wistar albino rats. Nigerian Journal of Pure and Applied Science. 2018;31(2):3192-3196.
Achuba FI. Modulation of crude oil induced alteration of oxidative stress indices in rat by red palm oil. Journal of Applied Science and Environmental Management. 2018;22(6):929-932. DOI: https://doi.org/10.4314/jasem.v22i6.15
Orisakwe OE, Akumkan DD, Njan AA et al. Testicular toxicity of Nigerian bonny light crude oil in male albino rat. Reproductive Toxicology 2004;18:439-442. DOI: https://doi.org/10.1016/j.reprotox.2004.02.002 [PMid:15082080]
Ovuru SS, Oruwari BM, Berepubo NA. Nephrotoxicity in rabbits following subchronic exposure to Nigerian crude oil. Nigerian Veterinary Journal. 2004;25(2):19-26. DOI: https://doi.org/10.4314/nvj.v25i2.3470
Maria VL, Correia AC, Santos MA et al. Biochemical and genotoxic responses to benzo[a]pyrene. Ecotoxicology and Environmental Safety. 2002;53:86-92. DOI: https://doi.org/10.1006/eesa.2002.2205 [PMid:12481863]
Vanzella TP, Martinez CBR, C'olus IMS. Genotoxic and mutagenic effects of diesel oil water soluble fraction on a neotropical fish species. Mutation Research. 2007;631:36-43 DOI: https://doi.org/10.1016/j.mrgentox.2007.04.004 [PMid:17509929]
Wilk A, Waligórski P, Lassak A et al. Polycyclic aromatic hydrocarbons-induced ROS accumulation enhances mutagenic potential of T-antigen from human polyoma virus JC. Journal of Cell Physiology. 2013;228(11):2127-2138. DOI: https://doi.org/10.1002/jcp.24375 [PMid:23558788 PMCid:PMC4007759]
Braham RP, Blazer VS, Shaw CH et al. Micronuclei and other erythrocyte nuclear abnormalities in fishes from the Great Lakes Basin, USA. Environmental and molecular mutagenesis. 2017;58(8):570-581. DOI: https://doi.org/10.1002/em.22123 [PMid:28868735 PMCid:PMC5656883]
Achuba FI. Effect of Vitamins C and E Intake on Blood Lipid Concentration, Lipid Peroxidation, Superoxide Dismutase and Catalase Activities in Rabbit Fed Petroleum Contaminated Diet. Pakistan Journal of Nutrition. 2005;4(5):330-335. DOI: https://doi.org/10.3923/pjn.2005.330.335
Uboh FE, Akpanabiatu MI, Alozie Y et al. Comparative effect of vitamins A and E on gasoline vapours-induced haematotoxicity and weight-loss in male rats. International Journal of Pharmacology. 2009;5(3):215-221. DOI: https://doi.org/10.3923/ijp.2009.215.221
Achuba FI, Awhin PE. Protective influence of antioxidant vitamins on hematological indices of rabbits fed crude-oil-contaminated diet. Toxicological and Environmental Chemistry. 2009;9(3):505-510. DOI: https://doi.org/10.1080/02772240802138069
Ita SO, Aluko EO, Atang DE et al. Vitamin C or E supplementation ameliorates Nigerian Bonny light crude oil-induced erythrocyte haemolysis in male Wistar rats. Biochemistry and Molecular Biology. 2013;1(3):44-51. DOI: https://doi.org/10.12966/bmb.10.01.2013
Achuba FI, Nwokogba CC. Effects of honey supplementation on hydrocarbon-induced kidney and liver damage in Wistar albino rats. Biokemistri. 2015;27(1):50-55.
Achuba FI, Obaremi C. Effects of Selenium Fortified Diet on Inflammatory Markers in Wistar Albino Rats Exposed to Crude Oil. Nigerian Journal of Pharmaceutical and Biomedical Research. 2018;3(3):209-216.
Achuba FI. Role of bitter leaf (Vernonia amygdalina) extracts in prevention of renal toxicity induced by crude petroleum contaminated diets in rats. International Journal of Veterinary Science and Medicine. 2018;6(2):172-177. DOI: https://doi.org/10.1016/j.ijvsm.2018.07.002 [PMid:30564592 PMCid:PMC6286397]
Bafor EE, Ukpebor F, Omoruyi O et al. Acute toxicological evaluations of the methanol leaf extract of Justicia flava (Vahl) Acanthaceae in mouse models. Trop J Nat Prod Res. 2019;3(4):138-144. doi.org/10.26538/tjnpr/v3i4.6 DOI: https://doi.org/10.26538/tjnpr/v3i4.6
Nowland MH, Hugunin KMS, Rogers KL. Effects of short-term fasting in male Sprague-Dawley rats. Comparative Medicine. 2011;61(2):138-144.
Wu B, Ootani A, Iwakiri R et al. T cell deficiency leads to liver carcinogenesis in azoxymethane-treated rats. Experimental Biology and Medicine. 2005;231:91-98. DOI: https://doi.org/10.1177/153537020623100111 [PMid:16380649]
Kegel V, Deharde D, Pfeiffer E et al. Protocol for isolation of primary human hepatocytes and corresponding major populations of non-parenchymal liver cells. Journal of Visual Experiments. 2016;(109):1-10. DOI: https://doi.org/10.3791/53069 [PMid:27077489 PMCid:PMC4841324]
Valente MJ, Henrique R, Costa VL et al. A rapid and simple procedure for the establishment of human normal and cancer renal primary cell cultures from surgical specimens. PLoS ONE. 20116;(5):e19337. DOI: https://doi.org/10.1371/journal.pone.0019337 [PMid:21573239 PMCid:PMC3087760]
Sharma AK, Sharma A. Chromosome technique theory and practice. London: Butter-Worths Publishers; c1980. 724p.
Duncan DB. Multiple ranges and multiple F Tests. Biometrics. 1955;11:1-10. DOI: https://doi.org/10.2307/3001478
Achuba FI. The effects of sublethal concentrations of crude oil on the growth and metabolism of cowpea (Vigna unguiculata) seedlings. Environmentalist. 2016;26(1):17-20. DOI: https://doi.org/10.1007/s10669-006-5354-2
Paz-y-Miño C, López-Cortés A, Arévalo M et al. Monitoring of DNA damage in individuals exposed to petroleum hydrocarbons in Ecuador. Annals New York Academy of Science. 2008;140:121-128. DOI: https://doi.org/10.1196/annals.1454.013 [PMid:18991910]
Elsayed ASI. DNA fragmentation and apoptosis caused by gasoline inhalation, and the protective role of green tea and Curcumin. Pyrex Journal of Biomedical Research. 2015;1(6):68-73.
Mzindle NB. Anti-inflammatory, Antioxidant and Wound-Healing Properties of Selected South African Medicinal Plants. MSc dissertation. 2017.
Kadiri HE, Asagba SO. The chronic effects of cyanide on oxidative stress indices in the domestic chicken (Gallus domesticus L.). The Journal of Basic and Applied Zoology. 2019;80:30 DOI: https://doi.org/10.1186/s41936-019-0098-y
Asagba SO, Kadiri HE, Ezedom T. Biochemical changes in diabetic rats treated with ethanolic extract of Chysophyllum albidum fruit-skin The Journal of Basic and Applied Zoology 2019; 80:42. https://doi.org/10.1186/s41936-019-0118-y DOI: https://doi.org/10.1186/s41936-019-0118-y
Achuba FI. Protective role of Elaesis guineensis leaves against crude oil tainted diet-induced hematotoxicity in Wistar rats. Iranian Journal of Toxicology. 2019;13(4):1-4.
Uboh FE, Ebong PE, Udosen EO. Effect of vitamin C on gasoline vapor-induced reproductive toxicity in female rats. Turkish Journal of Endocrinology and Metabolism. 2010;14:26-30.
Ufot SU, Luke UO, Uboh FE et al. Protective effect of ascorbic acid against Bonny Light crude oil induced atherosclerosis in rats. Research Journal of Environmental Toxicology. 2018;12:11-17. DOI: https://doi.org/10.3923/rjet.2018.11.17
Agyare C, Bempah SB, Boakye YD et al. Evaluation of antimicrobial and wound healing potential of Justicia flava and Lannea welwitschii. Evid Based Complement Alternat Med. 2013;2013:632927. DOI: https://doi.org/10.1155/2013/632927 [PMid:24159350 PMCid:PMC3789403]
Achuba FI, Osakwe SA. Petroleum induced free radical toxicity in Africa catfish (Clarias gariepinus). Fish Physiology and Biochemistry. 2003;29(2):97-103. DOI: https://doi.org/10.1023/B:FISH.0000035905.14420.eb
Dhamia KS, Nahi YY, Zainab AMA. Sperm DNA Fragmentation in Petroleum Workers. International Journal of Innovative Research in Science, Engineering and Technology. 2016;(6)5:0521-9529.
Uboh FE, Akpanabiatu MI, Atangwho IJ et al. Effect of Vitamin A on Weight Loss and Haematotoxicity Associated with Gasoline Vapours Exposure in Wistar Rats. International Journal of Pharmacology. 2008;4(1):40-45. DOI: https://doi.org/10.3923/ijp.2008.40.45
Uboh FE, Ebong PE, Akpan HD et al. Hepatoprotective effect of vitamins C and E against gasoline vapor-induced liver injury in male rats. Turkish Journal of Biology. 2012;(36):217-223.
Corrêa GM, de Alcântara AFC. Chemical constituents and biological activities of species of Justicia - a review. Brazilian Journal of Pharmacognosy. 2011;22(1):220-238. DOI: https://doi.org/10.1590/S0102-695X2011005000196
Panche AN, Diwan AD, Chandra SR. Flavonoids: an overview. Journal of Nutritional Science. 2016;5(e47):1-15. DOI: https://doi.org/10.1017/jns.2016.41 [PMid:28620474 PMCid:PMC5465813]
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