In 100 patients with chronic hepatitis C impaired transcription-translation apparatus was detected. The most expressed changes in cytogenetic parameters were observed in patients with chronic hepatitis C (CHC) and concomitant diabetes mellitus (DM).
The objective of the research was to detect changes in the functional state of the genotype (FSG) of the peripheral blood neutrophils (PBN) in patients with CHC and concomitant DM.
Materials and methods. The study involved 80 patients with CHC and concomitant DM at the age of 30 to 65 years (40 women and 40 men) with disease duration of 5 to 15 years. In addition to general methods of examination, the peculiarities of the FSG of the PBN were determined in all patients using the cytogenetic method of examination of the interphase nuclei.
Results. The chromatization index (CI) reduced in patients suffering from CHC and concomitant DM and those suffering from CHC without concomitant pathology in comparison with healthy persons (р>0.1). The nucleolar index (NI) reduced by 58.5% in those suffering from CHC and concomitant DM (р<0.001) in comparison with healthy persons. The number of pathologically changed nuclei increased by 2.4 times (р<0.001) in patients with CHC without concomitant pathology and by 2.8 times (р<0.001) in CHC patients with concomitant DM in comparison with the control group. The number of PCN was significantly higher in patients with CHC and concomitant DM than in patients with CHC without concomitant pathology (р<0.01). When analyzing the rate of the micronucleus (MN) index, we did not establish any clear difference in MN among patients with CHC and concomitant DM and patients with CHC without concomitant pathology (0.83±0.10) vs. (1.05±0.20) (р>0.1). No clear difference was also established in the number of MN in healthy persons, patients suffering from CHC with concomitant DM and patients with CHC without concomitant pathology (р>0.1).
Conclusions. Significant disorders of the FSG of the PBN in patients with CHC and concomitant DM in comparison with patients with CHC without concomitant pathology were proved. The disorders of the second stage of realization of genetic information were detected as a result of a reduction in CI and NI among all patients with CHC and concomitant DM and those with CHC without concomitant pathology (р>0.1). In all examined patients there was determined a tendency towards changes in the components of the cytogenetic status by the MN rate.
Hural AL, Maryevskyy VF, Serheeva TA, et al. Нepatitis C in Ukraine: epidemiological aspects of the problem. Suchasni infektsii. 2008;1:53-60.
Vorozhbit OB. Problem of extrahepatic lesions in chronic hepatitis C. Praktychna medytsyna. 2002;IX(3):97-101.
Neiko EM, Kovalchuk LYe, Chernyuk NV. Epigenetic mechanisms regulating the activity of genes and multifactorial disease. Halytskyi likarskyi visnyk. 2007;14(1):11-14.
Bochkova NP. Hereditary diseases: national guidelines. Bochkova NP, Ginter AK, Puzyrev VP, editors. GEOTAR – Media. Moscow. 2012;936.
Paliychuk IV, Kovalchuk LYe, Yastrebova OYa. Cytogenetic indicators of functional state of the neutrophil genome in the peripheral blood of patients with prosthetic stomatitis. Journal of Dentistry. 2010;3:59-64.
Popovich VI, Chernyuk NV, Kovalchuk LYe. Comprehensive assessment of cytogenetic, clinical and instrumental parameters in pathology of the upper respiratory tract and chronic obstructive pulmonary disease. Rynolohiia. 2006;1:3-9.
Kaminsky VYa. Evaluation of functional state of chromatin neutrophils in patients with chronic kidney disease, glomerulonephritis. Ukraiinskyi medychnyi almanakh. 2012;15(3):83-85.
Кovalchuk LE, Tsymbalista OL, Vovk ZV. Results of the study of functional state of neutrophils in the peripheral blood of children with iron deficiency anemia. Arkhiv klinichnoii medytsyny. 2007;2:35-37.
Virstyuk NH. Diagnostic value of cytogenetic indices in patients with chronic hepatitis. Halytskyi likarskyi visnyk. 2001;8(4):23-25.
Barylyak IR, Frolov VM, Pinskiy LL, et al. Cytogenetic disorders in patients with chronic hepatitis B and C. Tsitologiya i genetika. 2000;34(4):3-5.
Рinsky LL, Frolov VM. Relationships between cytogenetic and immunological parameters in patients with chronic hepatitis B. Virusnye gepatity s perenteralnym mekhamizmom peredachi vozbuditeley i ikh iskhody. Kiev. 2001;50-54.
Sakakura C, Hagivara A, Tanaguchi H, et al.Chromosomal aberrations in human hepatocellular carcinomas associated with hepatitis C virus infection detected by comparative genomic hybridization. Brit. J. Cancer. 1999; 80(12):2034-2039.
Boyer L, Blum HE, Maier KP, et al. Liver Cirrhosis and its Development. J . – Dordrecht. Boston. London. 2000;354.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.