Excretion of Ammonia and Urea by the Kidneys after Liver Resection and Hyperbaric Oxygenation



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Savilov, P. N., & Molchanov, D. V. (2015). Excretion of Ammonia and Urea by the Kidneys after Liver Resection and Hyperbaric Oxygenation. Galician Medical Journal, 22(3), 188-194. Retrieved from https://ifnmujournal.com/gmj/article/view/431


The objective of the study is to examine the impact of hyperbaric oxygenation (HBO) on ammonia excretion by kidneys in case of hepatectomy.

Materials and methods. Experiments were conducted on 138 mature (female) rats. HBO was conducted at 3 atm during 50 minutes (three times), one session daily after hepatectomy (15-20% of the organ weight). Test objects were kidneys, blood (aorta, v.renalis), urine. The concentration of ammonia, glutamine, and urea were identified.

Results. By eliminating post-surgery arterial hyperammonemia, HBO stimulates ammonia ions excretion with urine. It is a result of HBO's eliminating the inhibiting impact of hepatectomy on ammonia secretion into renal tubules and intracellular ammoniogenesis activation in nephrocytes, also not associated with “arterial” glutamine deamidation. At the same time HOT improves the activating hepatectomy impact on glutamine cycle in kidneys primarily due to increase in “renal glutamine” formation and its release into the blood flow. HOT increases the stimulating activity of hepatectomy on urea reabsorbtion in kidneys, but in contrast to non-oxygenated rats there is reduction in urea excretion with urine, due to the increase in urea concentration in arterial blood. Termination of hyperoxic activity over the body restores urea reabsorbtion in kidneys, with HOT stimulating activity over urea formation by nephrocytes and its release into the blood flow being preserved.



Detienne P. Kidney functions in Human Physiology (edited by R. Smidt, G. Tevs) Translated from English. М.: Mir, 2010; 3: 785-812.

Curthoys NP. Role of mitochondrial glutaminase in rat renal glutamine metabolism J.Nutr.2001; 131(9): 2491-2497.

Savilov PN, Molchanov DV. Kinetics of ammonia in the organiosm after liver resection and hyperbaric oxygenation General Pathology and Pathology Physiology Ukraina. Lugansk. 2010; 5(No.3): 114-118.

Kosenko ЕА, Kaminskiy YuG. Cellular mechanisms of ammonia toxicity М.: LKI, 2008.

Welborn TC. Alfa keto-glutarate, ornithin and growth hormone displace glutamine dependent ammoniogenesis and en chance renal base generation and function Clin. Nutr.-1993; 12(1): 49-50.

Silbernage S, Guder W. Molecular scifiticity of tubular rearbsorbtion of L-aspartate and L-glutamate. A microperfusion study in rat kidney Pflugers Arch.1978; 377(15): 231-240.

Guder WG, Wirtheuschu G. Metabolism of isolated kidney tubules. Interactioms between lactate, glutamine and oleate metabolism Eur. J. Biochem.1979; 99(3): 577-584.

Serov VV, Paltsev МА. Kidneys and arterial hypertension М.: Medicine, 1993

Savilov PN, Molchanov DV, Yakovlev VN. Nitrous metabolites kinetics in kidneys in case of hepatectomy Problems of boil., med. and pharm. chemistry 2012; 7: 43-47. [In Russ]

Molchanov DV, Savilov PN. Renal mechanisms elimination of ammonia liver resections (experimental study) Russian bulletin of children surgery, anaesthesiology and critical care medicine 2013; 3(3): 64-68. [In Russ]

Savilov PN, Ykovlev VN. Hyperbaric oxygenation and partial hepatectomy effects on liver bloodstream, oxegen pressure and ammonia detoxication in the liver under chronic hepatitis Jugosl. Physiol. Pharmacol. Acta 2006; 42(32): 103-114.

Savilov PN. Role and place of hyperbaric oxygenation in hepatic failure General Reanimatology 2009; 5(5): 72-79. [In Russ]

Savilov PN. Impact of hyperbaric oxygenation on nitric balance of gastrointesninal organs at hepatectomy: experimental study Anestesiology and Reanimatology 2005; 6: 59-62. [In Russ]

Savilov PN. Nitrous metabolism in spleen in case of hepatectomy and hyperbaric oxygen therapy. Biol. magagine. Armenia 2014; 66(2): 6-11.

Silakova AI, Trubin GP, Yavlikova AI. Micromethod for ammonia and glutamine identification in trichloroacetic tissue extracts. Medical chemistry 1962; 8(5): 538-544. [In Russ]

Keller H, Muller-Beisenritz M, Neumann E. Eine Methode zur Ammoniakbestimmung in Capillarblut Klin. Wsch.1967; 15: 314-319.

Harris M. Studies regenerating a glutamine-like substance in blood and spinal fluid, including a method for its quantitative determination J. Clin. Invest.1943; 22(4): 569-576.

Richterrich D. Clinical. Chemistry. N.Y.:Academia Press, 1962.

Normal and pathological cytology of the liver parenchyma (Срeissin E.M ed.) Leningrad: Science, 1969.

Kozlov ЕА, Kovalenko NA. Glutaminases Biological chemistry success M. 1972; 13: 49-79. [In Russ]

Häussinger D. Hepatocyte heterogeneity in glutamine and ammonia metabolism and the role of an intercellular glutamine cycle during ureogenesis in perfuzed rat liver Eur. J. Biochem 1983; 133: 269-275.

Savilov PN. Liver glutamic cycle after resection Problems of boil., med. and pharm. chemistry 2010; 7: 53-58. [In Russ]

Lomivorotov VV, Efremov CM, Shmirev VA, Ponomarev DN, Svyatchenko AV, Knyazkova LG. Glutemine cardioprotective effects in patients with ischemic heart disease operated under cardiopulmonary bypass Anestesiology and reanimatology 2012; 2: 14-18. [In Russ]

Savilov PN, Molchanov DV, Yakovlev VN. Impact of hyperbaric oxygenation on glutamine kinetics in hepatic failure (an experimental study) General Reanimatology 2012; 8(2): 20-27. [In Russ]

Verbitskaya VS, Dolgikh VT, Korpacheva OV, Ostroglyadova IA. Glutamine correction of morphofunctional disoders of the small band liver in the posttraumatic period of experimental cardiac contusion (an experimental study) General Reanimatology 2014; 10(2): 31-40.

Leonov AN. Hyperoxia. Adaptation. Sanogenesis Voronezh: Voronezh State Medical Academy named after N.N. Burdenko, 2006.

Sinichkin АА. Hyperbaric oxygen therapy: proteins, blood-tissue interfaces. Directoty in hyperbaric biology and medicine 2002; 10(1-4): 131-132.

Kudryashev BA., Lyapina LA. Heparin-urea complex and its physical and chemical properties. Medical chemistry 1975; 21(2): 165-168. [In Russ]

Savilov PN., Ivanina MS. Hyperbaric oxygen correction of hemostasis distortions in case of marginal hepatectomy Hyperbaric physiology and medicine 1996; 4:24. [In Russ]

Lukash AI., Vnukov VV., Ananyan АА., Milyutina NP., Kvasha PN. Metal-containing blood plasma compounds in hyperbaric oxygen therapy Rostov-on-Don: LOGOS: 1996.

Savilov PN. Effects of the hyperbaric afteraction and organism posthyperoxic state Directoty in hyperbaric biology and medicine 2006; 14(1-4): 21-51. [In Russ]

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