AbstractThe aim of the work – is to study the dynamics of peculiarities of morphophunctional changes in structural elements of arteries’ walls of muscular type under the influence of the general deep hypothermia. There were used 20 white mature adult outbred rats weighing 160-180 g. Cooling was performed in accordance with the patented methods. Euthanasia of the rats was carried out by an overdose of ether anesthesia. Collection of the material was performed during the 7th, 14th and 30th days of post-hypothermic period. Pieces of humeral, femoral, renal, superior mesenteric arteries and celiac trunk were carried to paraffin blocks according to the conventional methods. With the help of sliding microtome there were received sections of 5-8 microns thick with subsequent staining with hematoxylin and eosin, fuchsin according to the Hart (identification of elastic fibers), trichrome staining according to Masson (identification of collagenous fibers), alcian blue after Stidman (definition of glycosaminoglycans). Ultrastructural study of the material was performed on the electron microscope PEM-125K. During the 7th day degenerative changes of the vascular wall structural components predominate, which during the 14th day are combined with compensatory-adaptive manifestations. During the 30th day after the action of general deep hypothermia there are expressed intracellular regenerative processes. The perspective is to study further changes which occur in the main arteries of elastic and mixed types at different terms of post-hypothermic period with the purpose to find ways of their prevention and correction, which is dictated by medical and social value of this problem.
Babak OYa, Lisova HV. Morphological changes of the vascular wall of carotid arteries in patients with chronic glomerulonephritis. Medytsyna sohodni i zavtra. 2011; 4 (53): 45-48.
Bahriy MM, Slyvka VI. Obliterate thromboangitis (Buerger’s disease): pathomorphological changes in the vessels of the amputated lower limbs. Patolohiya. 2007; 4 (2): 76-80.
Voronkov LH, Shkurat IA, Besaha YeM. Structural changes of main arteries in patients with chronic heart failure. Krovoobih ta hemostaz. 2005; 3-4: 15-19.
Kuzminova NV. Morphological changes of the vascular wall in hypertensive patients depending on the nature of complications. Visnyk morfolohiyi. 2009; 15 (1): 150-154.
Loktionova SA, Kabakov AE. Phosphatase inhibitors prevent the dephosphorylation of HSP27, destruction of stress fibrils and change of morphology of endothelial cells in the depletion of ATP. Bulleten ekperimentalnoy biologii i meditsiny. 2001; 9: 350-353.
Makhanova NA. The effect of stress caused by cooling in an early postnatal ontogenesis, on the blood pressure and heart function in normal and hypertensive rats. Bulleten ekperimentalnoy biologii i meditsiny. 2000; 12: 660-663.
Nikishkova IN, Kutikov AE. Peculiarities of general moderate periodic cooling modeling in Wistar rats. Problemy kriobiologii. 2000; 2: 113-114.
Shutka BV, Popadynets OH, Zhurakivska OYa inventors; Shutka BV, Popadynets OH, Zhurakivska OYa assignee. Method for modeling general deep hypothermia in experiment. Ukaine patent UA 65225. 2004 Mar 15.
Slabyy OB, Hnatiuk MS, Kovalchuk LF. Morphometric evaluation of arterial remodeling characteristics of myocardium in toxic lesions. Ukrayinsky medychny almanakh. 2009; 12 (4): 176-179.
Strukov AI, Puchkov VS, Kaufman YaYa. Inflammation. In: Obshchaya patologiya cheloveka. Moscow: Meditsyna; 1990. p. 3-74.
Gansburgskiy AN, Yaltsev AV, Ovchinnikov NL, et al. Structure of a population of smooth myocytes of various parts of aorta. Morfologiya. 2006; 129 (4): 36.
Shutka BV, editor. General deep hypothermia. Ivano-Frankivsk; 2006. 300 p.
Yuryk II, Holovata TK. Histological changes of major vessels of the lower limbs of rats of immature and reproductive age under the conditions of hyperuricemia depending on the duration of the experiment. Visnyk VDNZU “Ukrainian Medical Dental Academy”. 2015; 4 (52): 286-290.
Aird WC. Endothelial cell heterogeneity. Crit Care Med. 2003; 31(4): 221-230. http://doi.org/10.1097/01.CCM.0000057847.32590.C1 PMid:12682444
Özhan H, Yazici M, Albayrak S, Erbilen E, Bulur S, Akdemir R, Uyan C. Elastic properties of the ascending aorta and left ventricular function in patients with hypothyroidism. Echocardiography. 2005; 22 (8): 649-656. http://doi.org/10.1111/j.0742-2822.2005.00189.x PMid:16174118
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