Pyo-inflammatory diseases are quite common among various surgical diseases in children. Etiotropic antimicrobial therapy of these conditions is of primary importance. Modern pathogenic bacteria possess a high degree of drug resistance to antimicrobials, which significantly reduces the effectiveness of treatment and contributes to the spread of infection and the development of pyo-inflammatory complications.
The objective of the research was to study peculiar properties of virulence factors production by S. aureus within 24 hours as well as to determine on this basis the directions of improving the efficiency of antimicrobial therapy of pyo-inflammatory diseases in children.
Materials and methods. There were conducted in vitro experimental microbiological studies concerning the study of the daily dynamics of virulence factors of causative agents of purulent-inflammatory diseases in children. The materials for the study were as follows: wound tissues, purulent exudate, dressings and suture material, catheters and drainage elements.
Results. Microbiological studies revealed that drug resistance of pathogenic microorganisms originates from the production of various factors of bacterial aggression including the formation of biofilms. The study showed that the level of production of virulence factors and the sensitivity of S. aureus to antimicrobial agents were not constant over the day. Recorded peaks of increasing adhesive activity, teichoic acids content, production of planktonic cells and formation of dense biofilms by S. aureus depended on the time of administration of antimicrobials and maximum concentration of the medication in an organism.
Conclusions. The regularity observed allowed us to develop a new concept to increase the efficiency of treatment of pyo-inflammatory diseases in children by means of change in the time for administration of antimicrobial agents during the day so that the period of maximum action would coincide with periods of minimal drug resistance of pathogens. This will allow us to significantly improve the effectiveness of antimicrobial therapy without increasing doses of medications that are administered.
Buharin OV, Perunova NB, Fadeev SB, et al. Bioritmy antibiotikorezistentnosti mikroorganizmov. Zhurn mikrobiol epidemiol immunobiol. 2008; 5:35–38.
Voznesenskij NA. Bioplenki – terapevticheskaja mishen' pri hronicheskih infekcijah. Pul'monologija i allergologija. 2008; 3:56–64.
Il'ina TS, Romanova JM, Gincburg AL. Bioplenki kak sposob sushhestvovanija bakterij v okruzhajushhej srede i organizme hozjaina: fenomen, geneticheskij kontrol' i sistemy reguljacii ih razvitija. Genetika. 2004; 40(11):1445–1456.
Mavrov II, Vasil'chenko VN, Belozerov AP. Bioplenki i Quorum sensing u mikroorganizmov. Bioplenki i problema jeffektivnosti antibakterial'noj terapii. Dermatologіja ta venerologіja. 2007; 4(38):19–22.
Moshkevich IR. Mikrobnye bioplenki pri smeshannyh infekcijah. Extended abstract PhD dissertation. Saint Petersburg; 2007. 20 p.
Tsiganenko AJ, Mіshyna MM, Kurbanov RA inventors; Hark nac med un-t assignee. Sposіb vіdtvorennja bіoplіvok mіkroorganіzmіv in vitro. Ukaine patent UA 47944. 2010 Feb 25.
Gisak SN, Bolysheva GS, Gagloev VM, et al. Polimorfizm gnojnoj infekcii u detej i rezul'taty ee postojannogo monitoringa. In: Sovremennye tehnologii v pediatrii i detskoj hirurgii. Proceedings of VI Rossijskij kongress; 2007, Oct 23–25; Moscow (Russia): Moscow; 2007. p.243–444.
Samarin DV, Juhymenko OO. Antybiotykorezystentnist' u hirurgii': mehanizmy formuvannja ta pidhody do vyznachennja. Hirurgija dytjachogo viku. 2012;2: 79–83.
Raffa RB, Iannuzzo JR, Levine DR, et al. Bacterial communication («quorum sensing») via ligands and receptors: a novel pharmacologic target for design of antibiotic drugs. J Pharmacol Exp Ther. 2005; 312(2):417–423. http://dx.doi.org/10.1124/jpet.104.075150 PMid:15528454
Bauer WD, Robinson JB. Disruption of bacterial quorum sensing by other organisms. Curr Opin Biotechnol. 2002;13:234–237. http://dx.doi.org/10.1016/S0958-1669(02)00310-5
Kumarasamy KK, Toleman MA, Walsh TR, et al. Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: a molecular, biological, and epidemiological study. Lancet Infect Dis. 2010;10(9):597–602. http://dx.doi.org/10.1016/S1473-3099(10)70143-2
Spellberg B, Guidos R, Gilbert D, et al. The epidemic of antibiotic-resistant infections: a call to action for the medical community from the Infectious Disease Society of America. Clin Infect Dis. 2008; 46(2):155–164. http://dx.doi.org/10.1086/524891 PMid:18171244
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.