This article represents the electronic-microscopic examination of blood vessels of the hemomicrocirculatory bed of the iliac lymph nodes of white rats, males of reproductive age, who, during six weeks, received narcotic opioid analgesic – nalbuphine, increasing the weekly dose of the medicine to create a model of physical opioid dependence according to Ukraine Patent #76564 U.
It was determined that opioid nalbuphine causes the reactive changes in the blood vessels of the hemomicrocirculatory bed of the iliac lymph nodes in the early stages of drug administration – within 1-2 weeks. A prolonged six-week administration of nalbuphine leads to profound destructive changes in the microvessels: the lumen of the hemocapillaries, which contains mainly the destructively altered erythrocytes, is expanding; the wall of microvessels, accompanied by hemorrhages into the vascular space, is violated; the structure of endotheliocytes nuclei changes, their cytoplasm swells, and organelles are damaged in it; the basement membrane thickens; the perivascular space swells and dilates. One week after the abolition of nalbuphine, the inverse changes in the blood vessels of hemomicrocirculatory bed do not occur.
Harapko TV. Peculiarities of microstructural changes of the lobules of the retrosternal gland of rats after four weeks of the opioid exposure to the body. Ukrayinsky naukovo-medychny molodizhny zhurnal. 2016; 1 (93): 19-23.
Harapko TV. Structural changes in the brain substance of thymus particles of white rats in six weeks' effect of opioid nalbuphine. Problemy klinichnoyi pediatriyi. 2016; 1-2 (31-32): 19-25.
Harapko TV, Holovatsky AU. Microscopic changes in the thymus of rats for a long-term effect of the opioid. Klinichna anatomiya ta operatyvna khirurhiya. 2016; 2 (56): 55-59.
Holovatsky AS, Valko OO. Morphofunctional changes in the lymph nodes under the influence of the chemical and physical factors on the body. Naukovy visnyk Uzhhorodskoho universytetu, seriya "Medytsyna". 2016; 1 (53): 131-136.
Klimenko NA, Sorokina IV, Savenko IA, Gorgol NI. Morphofunctional state of the thymus and spleen during inflammation against the background of the action of a non-selective blocker of opioid peptides of naloxone. Experymentalna i klinichna medytsyna. 2010; 1: 10-15.
Mayborodin IV, Streltsova EI, Zarubenkov OA, Egorov DV, Shevela AI. Lymphoid organs and cells in exposure to interleukin-2. Morfologiya. 2009; 135 (1): 62-66.
Lohash MV, Pokotylo PB, Fedevych YM, Kryvko YuYa. Changes of biochemical parameters of rat blood in the intoxication with opioids in the dynamics of the experiment. Klinichna ta Eksperymentalna Medytsyna. 2014; 2: 63-64.
Melnyk NO, Chekmareva IV, Chaikovsky YB. Reactive changes of organs of the immune system under the influence of pathological factors. Klinichna anatomiya ta operatyvna khirurhiya. 2004; 3 (3): 4-8.
Novytsky IYa, Yakymiv NYa, Yerokhova OM, Korol OO, Novytsky MI, Fitkalo OS. Toxic damage to the optic nerves due to the prolonged intake of levomitsetin against the background of drug dependence on Codterpin. Oftalmolohichny zhurnal. 2012; 3: 43-45.
Onysko RM, Paltov YeV, Fik VB, Vilkhova IV, Kryvko YuYa, Yakymiv NYa, Fitkalo OS, inventors. Method for the simulation of physical opioid dependence in rats. Lviv National Medical University named after Danylo Halytsky, patent holder. Patent # 76564; 2013.
Popyk PM, Mateshuk-Vatseba LR. Ultrastructural organization of the endocrine part and hemomicrocirculatory bed of pancreas in the conditions of the long-term influence of the opioid in the experiment. Klinichna anatomiya ta operatyvna khirurhiya. 2015; 2 (52): 72-76.
Radchenko TM. Gender peculiarities of the prevalence and clinical-psychopathological manifestations of opioid dependence in women. Ukrayinsky visnyk psykhonevrolohiyi. 2016; 24 (2): 78-81.
Yakymiv NYa. Morphological characteristics of the structures of the iris-corneal angina in different periods of action and in the early terms after abolition of experimental opioid influence. Oftalmologiya. Vostochnaya Evropa. 2014; 2: 89-97.
Zielińska M, Haddou TB, Cami-Kobeci G. Anti-inflammatory effect of dual nociceptin and opioid receptor agonist, BU08070, in experimental colitis in mice. European journal of pharmacology. 2015; 765: 582-590. DOI: https://doi.org/10.1016/j.ejphar.2015.09.021 [PMid:26404500 PMCid:PMC5064847]
Bailey CP, Connor M. Opioids: cellular mechanisms of tolerance and physical dependence.Curr. Opin. Pharmacol. 2005; 5(1):60-68. DOI: https://doi.org/10.1016/j.coph.2004.08.012 [PMid:15661627]
Maremmani I, Pacini M, Popovic D. Affective temperaments in heroin addiction. J. Affect. Disord. 2009; 117(3):186-192. DOI: https://doi.org/10.1016/j.jad.2009.01.007 [PMid:19201034]
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