Comparative Histologic Evaluation of Vertebrate Ovaries



How to Cite

Odokuma, E. I. (2018). Comparative Histologic Evaluation of Vertebrate Ovaries. Galician Medical Journal, 25(2).


Comparative Anatomy is a subspecialty of Anatomical Sciences that deals with/involves the possible existing structural relatedness in organisms. By careful description of specific structures, organs or systems, comparisons are made that draw similarities or differences between organs. The aim of this area in Anatomy was to provide evolutionary or adaptive explanations for detected characteristics – including maintaining the ancestral line.

Materials and Methods

Permission for this descriptive and observational study was obtained from the research and ethics committee of the Department of Human Anatomy and Cell Biology, Delta State University, Abraka (Number DELSU/CHS/ANA/118). Five female animals of the vertebrate class were used for the study (one from each vertebrate class). The animals included: cat fish (Clariasgariepinus); toad (Bufobufo); agama lizard (Agama agama); domestic chicken (Gallus gallusdomesticus) and the Wistar rat (Rattusnorvegicus).


All the studied vertebrates except mammals showed growth and maturation of several follicles confirming the characteristic of multiple gestation characteristic of these animals. The phases of growth were very similar in all of the studied organisms. These follicles were disposed in a fibrous stroma capable of influencing the activity of the ovary, Lacunae were only found in birds and mammals.


The index study revealed several significant findings especially the occurrence of multiple stages of germ cell development in the female vertebrate gonads. These observations provided a vivid histologic basis for the argument of a common ancestral origin of the animal phylum albeit against the background of extensive impact of adaptational factors.


Weedall GD and HALLN. Sexual reproduction and genetic exchange in parasitic protists. Parasitology. 2015; 142(Suppl 1): 120-127. DOI: [PMid:25529755 PMCid:PMC4413856]

Defalco T and Capel B. Gonad Morphogenesis in vertebrates: divergent means to a convergent End. Annul Rev Cell DevBiol. 2009; 25: 457-482. DOI: [PMid:19807280 PMCid:PMC4507502]

ObemierJA and Baldwin RL. "Establishing an appropriate period of acclamatization following transportation of laboratory animals. ILAR J. 2008;47(4):364-369. DOI:

Animal Research Ethics "A Handbook of USP researchers." 1st ed. Research Office publisher, University of South Pacific. 2009; 5.

Culling CF. Handbook of histopathology techniques 3rd ed. Butterworth and Co limited London 1974; 5: 73-105. [PMCid:PMC1646058]

Donoghue, P.C.J. andPurnell, M.A. The Evolutionary Emergence of Vertebrates From Among Their Spineless Relatives Evolution: Education and Outreach. 2009; (2) 2: 204-212. DOI:

Figueiredo MB, Santos AG, Travassos CM, MagalhaesBR.Oocyte organization and ovary maturation of the big eye tuna (Thunnusobesus) in the west tropical Atlantic Ocean. Collect. 2008; 62(2): 579-585.

Al-Hamdani HM. A study on embryonic development of Gambusiaaffinis(Baird and Girard). PhD thesis, University of Mosul. 1999

Cakici O, Ucuncu SI. Oocyte development in zebrafish, Daniorerio(Teleostei :cyprinidae). J. Fish. Aqat. Sci. 2007; 24: 137-141.

Rahemo IF and Al-Shatter NMS. Observations on reproductive organs and tissues of two freshwater cyprinid fishes. J of Med and Med Sci. 2012;3(12):764-773

Kanamadi RD and Saidapur SK Pattern of Ovarian Activity in the Indian Toad BufomelanostictusProc. Indian NatnSciAcad.1982; 48(3):307-316.

Saidapur SK and Nadkarni VB A study of female reproductive cycle in two species of frogs, Ranacyanophlyctis (Schn.) and Ranatigrina (Daud). Karnatak Univ. J Sci. 1974; 19:23-27.

Lofts B Reproduction; in Physiology of Amphibia II ed B lofts. New York: Academic Press. 1974; 107-219.

Guraya SS A comparative histochemical study of fish (Channamaruleus) and Amphibia (Bufostomaticus) oogenesis; Zeifschrift fur Zellforschung. 1965; 65: 662-700. DOI:

Follett BK and Redshaw MR. The Physiology of vitellogeniesis; in Physiology of Amphibia. Ed, lofts B, New York; Academic Press. 1974; 2: 219-309.

Brown DD and David IB. Specific gene amplication in oocytes. Oocyte contains extra-chromosomal replicas of the genes for ribosomal RNA. Science. 1968; 160: 272-280. DOI: [PMid:4867987]

Al-MukhtarKAK and Webb AC. An ultrastructural study of primordial germ cells oogonia and early oocytes in Xenopuslaevis. J EmbryolExpMorphol. 1971; 26: 195-217. [PMid:5168216]

Dumont JN. Oogenesis in Xenopuslaevis. I stages of oocyte development in laboratory maintained animals J Morphol. 1972:136:113-180. DOI: [PMid:4109871]

Machado-Santos C, Santana LNS, Vargas RF, Abidu-Figueiredo M, Brito-Gitirana L and Chagas MA Histological and Immunological study of the ovaries and oviducts of the Juvenile female of Caiman Lairostris (Crocodilia:Alligatoridae). Zoologia 2015; 32(5): 395-402. DOI:

Tumkiratiwong P, Meesuk W, Chanhome L, Aowphol A Reproductive patterns of captive male and female monocle cobra, Najakaouthia(Lesson, 1831). Zoological Studies. 2012; 51(5): 692-700.

Guraya SS Ovarian Follicles in Reptiles and Birds. Berlin, Springer-Verlag, 1989; 287.

Calderón ML, Pérez RG, PinillaMPL Morphology of the ovary of Caiman crocodilus. Annals of Anatomy. 2004; 186(1): 13-24. DOI:

Lance VA Reproductive-Cycle of the American Alligator. Integrative and Comparative Biology 1989; 29(3): 999-1018. DOI:

Uribe MC, Guillette JR LJ. Oogenesis and ovarian histology of the American alligator Alligatormississippiensis. J of Morphol2000; 245(3): 225-240. DOI:<225::AID-JMOR4>3.0.CO;2-M

Norris DO. Vertebrate endocrinology. Tokyo, Academic Press, 2007; 550.

Vieira S, Romero De Perez G, Ramýrez-Pinilla MP Ultrastructure of the ovarian follicles in the placentotrophic Andean Lizard of the genus Mabuya(Squamata: Scincidae).Journal of Morphology 2010; 271(6): 738-749. [PMid:20101729]

Calderón ML, Pérez RG, PinillaMPL. Morphology of the ovary of Caiman crocodilus. Annals of Anatomy. 2004; 186(1): 13-24. DOI:

Callebaut M. The ovarian chordolacunar system in birds.Archives De Biologie 1988; 99: 1-15.

Ceriani SA, Wyneken J Comparative morphology and sex identification of the reproductive system in formalin preserved sea turtle specimens. Zoology. 2008; 111(3): 179-187. DOI: [PMid:18234484]

Bull JJ, Vogt RC. Temperature-sensitive periods of sex determination in Emydid turtles. J. Exp. Zool. 1981; 218: 435-440. DOI: [PMid:7338727]

Wibbels T, Bull JJ, Crews D. Chronology and morphology of temperature-dependent sex determination. J. Exp. Zool. 1991; 260: 371-381. DOI: [PMid:1744617]

Johnson AL, Woods DC. Dynamics of avian ovarian follicle development: cellular mechanisms of granulosa cell differentiation. Gen Comp Endocrinol. 2009; 163:12-17. DOI: [PMid:19059411]

Johnson AL. The avain ovary and follicle development: some comparative and practical insights. Turk J Vet AnimSci. 2014; 38: 660-669. DOI:

Callebaut M. The ovarian chordolacunar system in birds. Achives De Biologie. 1988; 99:1-15.

Nilsson EE, Larsen G, Skinner MK. Roles of Gremlin 1 and Gremlin 2 in regulating ovarian primordial to primary follicle transition. Reproduction. 2014; 38: 660-669. DOI:

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