Histomorphological Effects of Cottonseed Oil on Testes in Adult Male Wistar Rats


cottonseed oil

How to Cite

Chris-Ozoko, L. E., Okpe, O., Iju, W. J., & Oyem, J. C. (2018). Histomorphological Effects of Cottonseed Oil on Testes in Adult Male Wistar Rats. Galician Medical Journal, 25(4). https://doi.org/10.21802/gmj.2018.4.2


Cottonseed oil is an extract from the seeds of cotton plant, that is rich in protein, carbohydrate and fiber.

         The objective of the research was to study the effect of cottonseed oil on the histology of the testis.

         Materials and methods. Twelve adult male Wistar rats weighing 200 – 250g, were randomly sorted into four groups (I - IV), each containing three animals. Cottonseed oil was administered orally to the experimental animals by means of an orogastric tube. The rats in Group I (the control group) received standard rat pellet and oral normal saline ad libitum only. The animals in Groups II, III, and IV received 0.2 ml, 0.6 ml and 1.0 ml of cotton seed oil per body weight respectively. On the 21st day, the animals were sacrificed by cervical dislocation. The testes were excised and, then, fixed with appropriate fixatives for routine tissue processing.

         Results. The results revealed an increase in the luminal diameter of seminiferous tubules, a decrease in the Leydig cell population, the disorientation of spermatogenic series and their detachment from the germinal epithelium.

         Conclusions. The present study demonstrated that oral administration of cottonseed oil to male rats displayed a deleterious effect on their testis by disrupting spermatogenesis and the histological architecture of the testis.



Kovacic P. Mechanism of drug and toxic actions of metabolic effects of the anti-fertility agent, gossypol, on various reproductive organs of male mice. Int J Androl. 2003;3:507-518.

Wang X, Plhak LC. Production, characterization, and application of anti-gossypol polyclonal antibodies. J Agric Food Chem. 2000;48:5109-5116. DOI: https://doi.org/10.1021/jf0006022 [PMid:11087445]

Chang MC, Gum ZP, Saksena SK. Effect of gossypol on the fertility of male rats, hamsters and rabbits. Contraception. 1980; 21:461-469. DOI: https://doi.org/10.1016/0010-7824(80)90011-6

Shepu X, Shudong S, Yanwan W et al. Anti-spermatogenic effect of gossypol on the germinal epithelium of rat testes. Sci Sin. 1980;23:642. [PMid:7403840]

Edwards JD. Total synthesis of gossypol. J Chem Soc. 1958;80:3798-3799. DOI: https://doi.org/10.1021/ja01547a084

Coutinho EM. Gossypol: a contraceptive for men. Contraception. 2002;65:259-263. DOI: https://doi.org/10.1016/S0010-7824(02)00294-9

Shandilya L, Clarkson TB, Adams MR, Lewis JC. Effects of gossypol on reproductive and endocrine functions of male cynomolgus monkeys (Macaca fascicularis). Biol Repro. 1982;27:241-252 DOI: https://doi.org/10.1095/biolreprod27.1.241

Oko R, Hrudka F. Gossypol-induced early and delayed-effects in the seminiferous epithelium of the adult-rat. Contraceptive Del Syst. 1984;5:335-356.

Liang SX, Pang SN, Dong RH, Dai RX. Radio Immunuoassay of Serum T and LH in male rat administered gossypol. Acta Biol Exp Sinica. 1981;14:91-97.

Liu BS. A tentative ideal of the use of cooking cotton seed oil for fertility control. Shanghai J Chin Med. 1957;6:43-47.

Wright J, Bissonnette F, Duchesne C et al. Psychological distress and infertility: men and women respond differently in vitro. Fertil Steril. 1991;55:100-108. DOI: https://doi.org/10.1016/S0015-0282(16)54067-9

Coulson PB, Snell RL, Parise C. Short-term gossypol: focus on reactive oxygen species and electron transfer. Curr Med Chem. 1993;10(24):2711-2718.

Messiha FS. Effect of gossypol on kinetics of mouse liver alcohol and aldehyde dehydrogenase. Gen Pharmacol. 1991;22(4):573-576. DOI: https://doi.org/10.1016/0306-3623(91)90058-E

Paim T, Viana P, Brandão E et al. Impact of feeding cottonseed coproducts on reproductive system of male sheep during peripubertal period. Sci Agric. 2015;73(6):489-497 DOI: https://doi.org/10.1590/0103-9016-2015-0377

Kenfack A, Chombong JK, Ngoula F et al. Effect of feeding cottonseed cake on male fertility in rabbit. Bang J Anim. Sci. 2015;44(1):16-20. DOI: https://doi.org/10.3329/bjas.v44i1.23123

Hardley MA, Young C, Lin, Dim M. Effects of gossypol on the reproductive system of male rats. J Androl. 1981;2:190-199. DOI: https://doi.org/10.1002/j.1939-4640.1981.tb00615.x

Neena S, Meenakshi G. Effect of crude cottonseed oil containing gossypol on fertility of male and estrous cycle of female Bandicota bengalensis Gray and Hardwicke. J App Anim Res. 2013;41(2):156-165. DOI: https://doi.org/10.1080/09712119.2012.738230

Burnett D, Crocker J. Specimen handling and preparation for routine diagnostic histopathology. In: The science of laboratory diagnosis. 2nd ed. John Wiley and sons, England. 2005;1:5-7.

Linxi L, Ying G, Haiqi C et al. Cell polarity, cell adhesion and spermatogenesis; role of cytoskeletons. Facility Rev. 2017;61565. doi: [10.12688/f1000research.11421.1]

Kim EB, Scott B, Sussan MB, Heddeven LB. Ganong's review of Medical Physiology. 24ed. TATA Mcgraw Hill. 2010;419-420.

Al- Agha O, Axiotis C. An in-depth look at Leydig cell tumour of the testis. Arch Pathol Lab Med. 2007;131(2):311- 317.

Sharma S, Hanukoglu A, Hanukoglu I. Localization of epithelial sodium channel (ENaC) and CFTR in the germinal epithelium of the testis, Sertoli cells and spermatozoa. J Mol Histol. 2018;49(2):195-208. DOI: https://doi.org/10.1007/s10735-018-9759-2

Chenoweth PJ, Risco CA, Larsen RE et al. Effects of dietary gossypol on aspects of semen quality, sperm morphology and sperm production in young Brahman bulls. Theriogenology. 1994;42(1):1-13. DOI: https://doi.org/10.1016/0093-691X(94)90657-5

Hassan ME, Smith GW, Ott RS et al. Reversibility of the reproductive toxicity of gossypol in peripubertal bulls. Theriogenology. 2004;61(6):1171-1179. DOI: https://doi.org/10.1016/j.theriogenology.2003.07.007 [PMid:15037004]

El-Sharaky S, Newairy AA, Elguindy NM, Elwafa AA. Spermatotoxicity, biochemical changes and histological alteration induced by gossypol in testicular and hepatic tissues of male rats. Food Chem Toxicol. 2010;48(12):3354-3361. DOI: https://doi.org/10.1016/j.fct.2010.09.004

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