@article{Rieznichenko_Doroshenko_Dybkova_Gruzina_Ulberg_Chekman_2014, title={Estimation of iron nanoparticles’ substance biosafety in vitro and in vivo}, volume={21}, url={https://ifnmujournal.com/gmj/article/view/282}, abstractNote={<p><em>The aim of the work </em>was to estimate biosafety of iron nanoparticles’ substance according to <em>in vitro</em> and <em>in vivo </em>tests. <em>Materials and Methods:</em> biosafety of the substance of 40 nm sized spherical zero-valent iron nanoparticles has been established <em>in vitro</em> using cytotoxicity and genotoxicity tests. Biosafety <em>in vivo</em> has been determined according to the acute toxicity parameter (LD<sub>50</sub>) and the effect on cardiac function and state of rabbits’ hemodynamics. <em>Results</em>: there were no cytotoxic and genotoxic effects of iron nanoparticles’ substance on the test culture of eukaryotic cells. In case of intravenous injection of nanoparticles the average LD<sub>50</sub> for BALB/с mice was 220.3 mg/kg. Iron nanoparticles’ substance injected intravenously was safe according to hemodynamic parameters. <em>Conclusion</em>: obtained data on biosafety estimation denotes low level of potential risks associated with iron nanoparticles’ substance.</p&gt;}, number={3}, journal={Galician Medical Journal}, author={Rieznichenko, L. S. and Doroshenko, A. M. and Dybkova, S. M. and Gruzina, T. G. and Ulberg, Z. R. and Chekman, I. S.}, year={2014}, month={Sep.}, pages={87-90} }